USED SARTORIUS / ESSEN / INTELLICYT KSEP® 400 (KSEP400)

The Sartorius Ksep® 400 is an advanced automated single-use counterflow centrifugation system designed for cell harvesting, clarification, and washing in biopharmaceutical manufacturing.

Using patented counterflow technology, the system balances centrifugal force and fluid flow to retain cells in a fluidized bed while continuously processing media. This allows gentle, low-shear separation, preserving cell viability and maximizing product recovery.

The Ksep 400 is widely used in cell therapy, vaccine production, and recombinant protein manufacturing, offering a closed, GMP-compliant, and scalable solution from development to pilot-scale production.
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Key Highlights

- Automated single-use centrifugation system
- Patented counterflow centrifugation technology
- Closed system reduces contamination risk (cGMP ready)
- Low shear processing maintains high cell viability
- Handles very high cell densities (>150M cells/mL)
- Continuous processing (no stop discharge) capability
- High recovery rates (>90–95% typical)
- Compatible with single-use sterile consumables (Class VI)
- Scalable platform (development → pilot scale)
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Applications / Uses

- Cell harvesting & clarification
- Cell therapy manufacturing (CAR-T, MSC, iPSC, NK cells)
- Vaccine production
- Monoclonal antibody (mAb) processing
- Blood processing
- Microcarrier separation & washing
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Typical Specifications (Summary)

- Technology: Counterflow centrifugation
- Max G-Force: ~1,000 g
- Max Flow Rate: ~114 L/hr
- Chamber Volume: 400 mL (4 × 100 mL)
- Cell Capacity / Cycle: Up to ~80 billion cells
- Processing Volume: ~10 – 200 L (up to 500 L depending on process)
- Dimensions (W×D×H): ~107.5 × 72 × 140 cm
- Weight: ~350 kg
- Power: 208–240 V, single phase, 20A
- Flow Path: Fully single-use, sterile
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Typical Configuration Includes

- Ksep 400 main unit
- Integrated control system (touchscreen/HMI)
- Pump & centrifuge module
- Tubing & single-use flow path (varies)
- Data connectivity (USB / Ethernet / LAN)
- Consumable kit compatibility

Sameer Khanna

Sales Manager - BionexScientificUSA

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