The Agilent Seahorse XFe96 Analyzer is a high-throughput, real-time cell metabolic analyzer that measures key parameters of cellular bioenergetics, including mitochondrial respiration and glycolysis. Using 96-well microplates, it enables researchers to simultaneously evaluate the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells, providing crucial insights into metabolic function and energy production.
Includes
- System software
Specifications
- Throughput: 96 wells per plate
- Parameters Measured: Oxygen Consumption Rate (OCR) and Extracellular Acidification Rate (ECAR)
- Sample Type: Adherent or suspension cells, isolated mitochondria
- Assay Volume: 180–200 µL per well
- Measurement Method: Fluorescent biosensors for O₂ and pH
- Data Output: Real-time metabolic profiles and kinetic curves
- Operating Temperature: 15–40 °C (controlled environment)
- Power Requirements: 100–240 V AC, 50/60 Hz
Applications
- Mitochondrial function and bioenergetic analysis
- Drug discovery and metabolic toxicity screening
- Cancer cell metabolism and glycolytic shift studies
- Stem cell differentiation and energy pathway analysis
- Immunometabolism and disease modeling researchShow moreShow lessClick to Request More Info
