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The Cytiva ÄKTA Avant 150 is a premium, fully automated, scalable chromatography system designed for process development and purification of biomolecules, widely used for protein purification in research and bioprocess development. Engineered by Cytiva (formerly GE Healthcare Life Sciences), it supports both research-scale and pilot-scale purification processes, with a flow rate up to 150 mL/min and high-pressure operation capability up to 20 MPa, making it ideal for downstream process optimization and method development.
The ÄKTA Avant 150 is equipped with UNICORN™ software, offering powerful method development tools, real-time monitoring, and complete control over the purification workflow. The system features integrated UV, conductivity, pH, and pressure sensors, as well as flexible inlet and outlet valve configurations to accommodate a wide range of columns and applications.
Either used for affinity chromatography, ion exchange, gel filtration, or hydrophobic interaction chromatography, the ÄKTA Avant 150 provides high-resolution purification with exceptional reproducibility and scalability, bridging the gap between laboratory research and production environments.
The Cytiva AKTA AVANT 150 is a powerful liquid chromatography system designed specifically for biopharmaceutical process development and scale-up applications. This versatile instrument is engineered to deliver precise, reproducible results while supporting a wide range of purification techniques. Laboratories focused on protein purification and biomolecular separation trust the Cytiva AKTA AVANT 150 for its advanced features and user-friendly design.
One of the standout features of the Cytiva AKTA AVANT 150 is its impressive flow rate range, spanning from 0.01 to 150 mL per minute. This flexibility allows users to perform everything from small-scale scouting runs to larger pilot-scale purifications on the same system. The Cytiva AKTA AVANT 150 supports multiple chromatography modes including affinity, ion exchange, hydrophobic interaction, and size exclusion chromatography, making it adaptable to many purification workflows.
Central to the Cytiva AKTA AVANT 150 is its seamless integration with the UNICORN software platform. This software empowers users to automate purification methods, monitor runs in real time, and analyze data comprehensively. The Design of Experiments (DoE) feature built into UNICORN makes the Cytiva AKTA AVANT 150 an excellent choice for optimizing protocols, saving time and resources by systematically exploring key parameters.
The system is equipped with up to 18 buffer inlets, expandable to 46, which allows for complex gradient elutions and multiple buffer exchanges without manual intervention. Additionally, the Cytiva AKTA AVANT 150 accommodates up to 7 sample inlets, providing flexibility to handle various feedstocks or sequential sample injections in a single workflow. These features contribute to efficient, high-throughput purification processes.
Detection capabilities on the Cytiva AKTA AVANT 150 are broad and precise. Its UV detector operates across wavelengths from 190 to 700 nm, making it suitable for a wide variety of biomolecules. The system also includes pH and conductivity monitoring, ensuring that purification conditions remain tightly controlled throughout each run. This helps maintain product quality and improves reproducibility across batches.
A notable addition to the Cytiva AKTA AVANT 150 is the BufferPro module. This automated buffer preparation system reduces manual labor and error by preparing buffers during the run itself. It supports both isocratic and gradient elution modes, enhancing flexibility while simplifying workflows. BufferPro helps laboratories save time and increases consistency, which is crucial for process development.
Designed for durability and ease of maintenance, the Cytiva AKTA AVANT 150 features a compact and modular build. This makes it simple to service, upgrade, or customize according to specific project needs. The system is compatible with a wide range of chromatography columns and consumables, allowing users to select the best tools for their purification challenges.
The Cytiva AKTA AVANT 150 is especially valuable for labs transitioning from lab-scale purification methods to pilot-scale processes. Its ability to replicate methods with minimal adjustments means researchers can efficiently scale up without compromising performance. This system bridges the gap between discovery and manufacturing phases, accelerating bioprocess development.
In summary, the Cytiva AKTA AVANT 150 offers a combination of versatility, automation, and precision that makes it a cornerstone for biopharmaceutical purification workflows. Whether you are developing new protocols, scaling up production, or optimizing existing processes, this system provides the reliability and performance needed to achieve your goals.
Read about the User Manual of AKTA AVANT here: User Manual
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