USED THERMO FISHER SCIENTIFIC HYPERFORMA G3LITE 250 L SINGLE-USE BIORE

The Thermo Scientific™ HyPerforma™ G3Lite 250 L Single-Use Bioreactor is an integrated stirred-tank bioreactor designed for process development, pilot-scale production, and GMP biopharmaceutical manufacturing. The system combines a reusable stainless-steel vessel with a disposable HyPerforma BioProcess Container (BPC) and the G3Lite™ bioreactor controller, providing reliable process control while eliminating cleaning and sterilization requirements.

The 250 L system incorporates Thermo Fisher's proven single-use mixing technology with a dual-sparger design, optimized impeller geometry, and advanced TruBio™ automation for precise control of pH, dissolved oxygen (DO), temperature, agitation, and gas flow. It is widely used for mammalian cell culture, monoclonal antibody production, vaccines, recombinant proteins, and cell therapy manufacturing.

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Key Highlights

- 250 L single-use stirred-tank bioreactor
- Integrated G3Lite™ bioprocess controller
- Disposable HyPerforma BioProcess Container (BPC)
- 5:1 turndown ratio (50–250 L working volume)
- Dual-sparger design for efficient oxygen transfer and CO₂ stripping
- TruBio™ software with touchscreen HMI
- Automated control of pH, DO, temperature, agitation, and gas flow
- SmartParts™, SmartSensors™, and SmartScale™ technology
- Open-architecture controller for scalable bioprocessing
- Suitable for GMP and non-GMP manufacturing environments
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Applications / Uses

- Mammalian cell culture
- Monoclonal antibody production
- Recombinant protein manufacturing
- Vaccine production
- Cell and gene therapy process development
- Process optimization and scale-up
- Clinical manufacturing
- Biopharmaceutical research and production
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Typical Specifications (Summary)

(May vary depending on configuration)

- Bioreactor Type: Single-use stirred-tank bioreactor
- Working Volume: 50–250 L
- Total Vessel Volume: Approximately 316 L
- Controller: HyPerforma™ G3Lite™
- Agitation: AC or DC motor-driven impeller
- Temperature Control: Jacketed vessel with TCU/heater blanket
- Gas Control: Six mass flow controllers (Air, O₂, N₂, CO₂)
- Pumps: Four integrated Watson-Marlow peristaltic pumps (expandable)
- Sensors: pH, DO, temperature, pressure, foam, and weight
- Software: TruBio™ automation platform with Emerson DeltaV® architecture
- Operating Temperature: 5–40°C
- Electrical: 120/240 V (configuration dependent)
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Typical Configuration Includes

- HyPerforma™ 250 L stainless-steel bioreactor vessel
- G3Lite™ controller
- HyPerforma BioProcess Container (BPC)
- Integrated agitation system
- Temperature control unit (TCU)
- Gas control module
- Peristaltic pump system
- Touchscreen HMI
- Sensor interfaces
- Documentation and manualsShow moreShow lessClick to Request More Info

Type: Bioreactors